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Myonuclear transcriptional dynamics in response to exercise following satellite cell depletion.
- Source :
-
IScience [iScience] 2021 Jul 10; Vol. 24 (8), pp. 102838. Date of Electronic Publication: 2021 Jul 10 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds of myonuclei. Using a progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high-resolution characterization of myonuclear transcription, we show myonuclear functional specialization in muscle. After 4 weeks of exercise training, snRNA-seq reveals that resident muscle stem cells, or satellite cells, are activated with acute exercise but demonstrate limited lineage progression while contributing to muscle adaptation. In the absence of satellite cells, a portion of nuclei demonstrates divergent transcriptional dynamics associated with mixed-fate identities compared with satellite cell replete muscles. These data provide a compendium of information about how satellite cells influence myonuclear transcription in response to exercise.<br />Competing Interests: The authors declare no competing interests.<br /> (© 2021 The Authors.)
Details
- Language :
- English
- ISSN :
- 2589-0042
- Volume :
- 24
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- IScience
- Publication Type :
- Academic Journal
- Accession number :
- 34368654
- Full Text :
- https://doi.org/10.1016/j.isci.2021.102838