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Amber Extract Reduces Lipid Content in Mature 3T3-L1 Adipocytes by Activating the Lipolysis Pathway.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2021 Jul 30; Vol. 26 (15). Date of Electronic Publication: 2021 Jul 30. - Publication Year :
- 2021
-
Abstract
- Amber-the fossilized resin of trees-is rich in terpenoids and rosin acids. The physiological effects, such as antipyretic, sedative, and anti-inflammatory, were used in traditional medicine. This study aims to clarify the physiological effects of amber extract on lipid metabolism in mouse 3T3-L1 cells. Mature adipocytes are used to evaluate the effect of amber extract on lipolysis by measuring the triglyceride content, glucose uptake, glycerol release, and lipolysis-related gene expression. Our results show that the amount of triacylglycerol, which is stored in lipid droplets in mature adipocytes, decreases following 96 h of treatment with different concentrations of amber extract. Amber extract treatment also decreases glucose uptake and increases the release of glycerol from the cells. Moreover, amber extract increases the expression of lipolysis-related genes encoding perilipin and hormone-sensitive lipase (HSL) and promotes the activity of HSL (by increasing HSL phosphorylation). Amber extract treatment also regulates the expression of other adipocytokines in mature adipocytes, such as adiponectin and leptin. Overall, our results indicate that amber extract increases the expression of lipolysis-related genes to induce lipolysis in 3T3-L1 cells, highlighting its potential for treating various obesity-related diseases.
- Subjects :
- 3T3-L1 Cells
Adipocytes cytology
Adipocytes metabolism
Adiponectin genetics
Adiponectin metabolism
Amber chemistry
Animals
Cell Differentiation
Complex Mixtures chemistry
Ethanol chemistry
Glucose metabolism
Glycerol metabolism
Hypolipidemic Agents chemistry
Leptin genetics
Leptin metabolism
Lipid Droplets chemistry
Lipid Droplets drug effects
Lipid Droplets metabolism
Mice
Perilipin-1 genetics
Perilipin-1 metabolism
Phosphorylation drug effects
Sterol Esterase genetics
Sterol Esterase metabolism
Triglycerides metabolism
Adipocytes drug effects
Amber pharmacology
Complex Mixtures pharmacology
Gene Expression Regulation drug effects
Hypolipidemic Agents pharmacology
Lipolysis drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 26
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 34361783
- Full Text :
- https://doi.org/10.3390/molecules26154630