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Rapid Detection and Quantification of Patulin and Citrinin Contamination in Fruits.
- Source :
-
Molecules (Basel, Switzerland) [Molecules] 2021 Jul 27; Vol. 26 (15). Date of Electronic Publication: 2021 Jul 27. - Publication Year :
- 2021
-
Abstract
- Patulin (PAT) and citrinin (CTN) are the most common mycotoxins produced by Penicillium and Aspergillus species and are often associated with fruits and fruit by-products. Hence, simple and reliable methods for monitoring these toxins in foodstuffs are required for regular quality assessment. In this study, we aimed to establish a cost-effective method for detection and quantification of PAT and CTN in pome fruits, such as apples and pears, using high-performance liquid chromatography (HPLC) coupled with spectroscopic detectors without the need for any clean-up steps. The method showed good performance in the analysis of these mycotoxins in apple and pear fruit samples with recovery ranges of 55-97% for PAT and 84-101% for CTN, respectively. The limits of detection (LOD) of PAT and CTN in fruits were 0.006 µg/g and 0.001 µg/g, while their limits of quantification (LOQ) were 0.018 µg/g and 0.003 µg/g, respectively. The present findings indicate that the newly developed HPLC method provides rapid and accurate detection of PAT and CTN in fruits.
- Subjects :
- Aspergillus metabolism
Chromatography, High Pressure Liquid economics
Cost-Benefit Analysis
Data Accuracy
Food Quality
Limit of Detection
Penicillium metabolism
Time Factors
Chromatography, High Pressure Liquid methods
Citrinin analysis
Food Contamination analysis
Fruit chemistry
Malus chemistry
Patulin analysis
Pyrus chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1420-3049
- Volume :
- 26
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Molecules (Basel, Switzerland)
- Publication Type :
- Academic Journal
- Accession number :
- 34361698
- Full Text :
- https://doi.org/10.3390/molecules26154545