Effective Detection and Monitoring of Glioma Using [ 18 F]FPIA PET Imaging.

Background: Reprogrammed cellular metabolism is a cancer hallmark. In addition to increased glycolysis, the oxidation of acetate in the citric acid cycle is another common metabolic phenotype. We have recently developed a novel fluorine-18-labelled trimethylacetate-based radiotracer, [ ¹⁸ F]fluoro-pivalic acid ([ ¹⁸ F]FPIA), for imaging the transcellular flux of short-chain fatty acids, and investigated whether this radiotracer can be used for the detection of glioma growth.
Methods: We evaluated the potential of [ ¹⁸ F]FPIA PET to monitor tumor growth in orthotopic patient-derived (HSJD-GBM-001) and cell line-derived (U87, LN229) glioma xenografts, and also included [ ¹⁸ F]FDG PET for comparison. We assessed proliferation (Ki-67) and the expression of lipid metabolism and transport proteins (CPT1, SLC22A2, SLC22A5, SLC25A20) by immunohistochemistry, along with etomoxir treatment to provide insights into [ ¹⁸ F]FPIA uptake.
Results: Longitudinal PET imaging showed gradual increase in [ ¹⁸ F]FPIA uptake in orthotopic glioma models with disease progression ( p < 0.0001), and high tumor-to-brain contrast compared to [ ¹⁸ F]FDG ( p < 0.0001). [ ¹⁸ F]FPIA uptake correlated positively with Ki-67 ( p < 0.01), SLC22A5 ( p < 0.001) and SLC25A20 ( p = 0.001), and negatively with CPT1 ( p < 0.01) and SLC22A2 ( p < 0.01). Etomoxir reduced [ ¹⁸ F]FPIA uptake, which correlated with decreased Ki-67 ( p < 0.05).
Conclusions: Our findings support the use of [ ¹⁸ F]FPIA PET for the detection and longitudinal monitoring of glioma, showing a positive correlation with tumor proliferation, and suggest transcellular flux-mediated radiotracer uptake.