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Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids.
- Source :
-
Nature communications [Nat Commun] 2021 Aug 05; Vol. 12 (1), pp. 4730. Date of Electronic Publication: 2021 Aug 05. - Publication Year :
- 2021
-
Abstract
- Brain organoids derived from human pluripotent stem cells provide a highly valuable in vitro model to recapitulate human brain development and neurological diseases. However, the current systems for brain organoid culture require further improvement for the reliable production of high-quality organoids. Here, we demonstrate two engineering elements to improve human brain organoid culture, (1) a human brain extracellular matrix to provide brain-specific cues and (2) a microfluidic device with periodic flow to improve the survival and reduce the variability of organoids. A three-dimensional culture modified with brain extracellular matrix significantly enhanced neurogenesis in developing brain organoids from human induced pluripotent stem cells. Cortical layer development, volumetric augmentation, and electrophysiological function of human brain organoids were further improved in a reproducible manner by dynamic culture in microfluidic chamber devices. Our engineering concept of reconstituting brain-mimetic microenvironments facilitates the development of a reliable culture platform for brain organoids, enabling effective modeling and drug development for human brain diseases.<br /> (© 2021. The Author(s).)
- Subjects :
- Animals
Brain cytology
Culture Media
Electrophysiological Phenomena
Extracellular Matrix physiology
Feasibility Studies
Gene Expression Profiling
Humans
Hydrogels
Induced Pluripotent Stem Cells cytology
Induced Pluripotent Stem Cells physiology
Models, Anatomic
Models, Neurological
Neurogenesis genetics
Neuroglia cytology
Neuroglia physiology
Organ Culture Techniques instrumentation
Organ Culture Techniques methods
Organoids cytology
Swine
Brain growth & development
Brain physiology
Lab-On-A-Chip Devices
Neurogenesis physiology
Organoids growth & development
Organoids physiology
Subjects
Details
- Language :
- English
- ISSN :
- 2041-1723
- Volume :
- 12
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature communications
- Publication Type :
- Academic Journal
- Accession number :
- 34354063
- Full Text :
- https://doi.org/10.1038/s41467-021-24775-5