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A protocol to visualize cytosolic aggresome-like bodies using confocal microscopy.
- Source :
-
STAR protocols [STAR Protoc] 2021 Jul 17; Vol. 2 (3), pp. 100674. Date of Electronic Publication: 2021 Jul 17 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Ubiquitin stress-induced NEDDylation leads to the formation of aggresome-like bodies (ALBs) in the perinuclear region of cells. Therefore, imaging analysis is essential for characterizing the biological phenotypes of ALBs. Here, we describe a protocol to monitor ALBs induced by ubiquitin stress using immunocytochemistry and to quantify cells containing ALBs. This optimized protocol details the use of readily available materials and reagents and can be applied to explore diverse molecules involved in stress-induced ALBs. For complete details on the use and execution of this protocol, please refer to Kim et al. (2021).<br />Competing Interests: The authors declare no competing interests.<br /> (© 2021 The Authors.)
- Subjects :
- Fluorescent Antibody Technique
HeLa Cells
Histone Deacetylase 6 immunology
Histone Deacetylase 6 metabolism
Humans
Immunohistochemistry methods
Inclusion Bodies metabolism
NEDD8 Protein immunology
NEDD8 Protein metabolism
Ubiquitin metabolism
Cytosol
Microscopy, Confocal methods
Molecular Biology methods
Subjects
Details
- Language :
- English
- ISSN :
- 2666-1667
- Volume :
- 2
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- STAR protocols
- Publication Type :
- Academic Journal
- Accession number :
- 34337443
- Full Text :
- https://doi.org/10.1016/j.xpro.2021.100674