CRISPR Interference of Adenylate Cyclases from Mycobacterium tuberculosis .

Authors :: Nadolinskaia NI
Zamakhaev MV
Shumkov MS
Armianinova DK
Karpov DS
Goncharenko AV
Source :: Applied biochemistry and microbiology [Appl Biochem Microbiol] 2021; Vol. 57 (4), pp. 421-425. Date of Electronic Publication: 2021 Jul 27.
Publication Year :: 2021
Abstract: This work describes a modification of the pRH2521 vector of the pRH2502/pRH2521 system for CRISPR-dCas9-mediated RNA interference. The modification enabled an increase in the cloning efficiency of guide RNA spacers. The ability of the modified pRH2502/pRH2521 system to suppress the transcription of certain genes was evaluated with the use of genes of Mycobacterium tuberculosis adenylate cyclases. The results revealed the limitations of the pRH2502/pRH2521 system for CRISPR interference associated with the probability of the detection of a protospacer adjacent motif (PAM) in the gene promoter region.<br />Competing Interests: The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.<br /> (© Pleiades Publishing, Inc. 2021, ISSN 0003-6838, Applied Biochemistry and Microbiology, 2021, Vol. 57, No. 4, pp. 421–425. © Pleiades Publishing, Inc., 2021.Russian Text © The Author(s), 2021, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2021, Vol. 57, No. 4, pp. 326–331.)