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Physical interactions between MCM and Rad51 facilitate replication fork lesion bypass and ssDNA gap filling by non-recombinogenic functions.
- Source :
-
Cell reports [Cell Rep] 2021 Jul 27; Vol. 36 (4), pp. 109440. - Publication Year :
- 2021
-
Abstract
- The minichromosome maintenance (MCM) helicase physically interacts with the recombination proteins Rad51 and Rad52 from yeast to human cells. We show, in Saccharomyces cerevisiae, that these interactions occur within a nuclease-insoluble scaffold enriched in replication/repair factors. Rad51 accumulates in a MCM- and DNA-binding-independent manner and interacts with MCM helicases located outside of the replication origins and forks. MCM, Rad51, and Rad52 accumulate in this scaffold in G1 and are released during the S phase. In the presence of replication-blocking lesions, Cdc7 prevents their release from the scaffold, thus maintaining the interactions. We identify a rad51 mutant that is impaired in its ability to bind to MCM but not to the scaffold. This mutant is proficient in recombination but partially defective in single-stranded DNA (ssDNA) gap filling and replication fork progression through damaged DNA. Therefore, cells accumulate MCM/Rad51/Rad52 complexes at specific nuclear scaffolds in G1 to assist stressed forks through non-recombinogenic functions.<br />Competing Interests: Declaration of interests The authors declare no competing interests.<br /> (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)
- Subjects :
- Cell Cycle genetics
Cell Nucleus metabolism
DNA Damage genetics
DNA Repair genetics
Methyl Methanesulfonate
Models, Biological
Protein Binding
Rad52 DNA Repair and Recombination Protein metabolism
Solubility
DNA Replication
DNA, Single-Stranded metabolism
Homologous Recombination genetics
Multiprotein Complexes metabolism
Rad51 Recombinase metabolism
Saccharomyces cerevisiae metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 2211-1247
- Volume :
- 36
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 34320356
- Full Text :
- https://doi.org/10.1016/j.celrep.2021.109440