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Zinc finger antiviral protein (ZAP) inhibits small ruminant morbillivirus replication in vitro.
- Source :
-
Veterinary microbiology [Vet Microbiol] 2021 Sep; Vol. 260, pp. 109163. Date of Electronic Publication: 2021 Jun 23. - Publication Year :
- 2021
-
Abstract
- Small ruminant morbillivirus (SRMV) is a highly contagious and economically important viral disease of small domestic and wild ruminants. Difficulty with its stable proliferation in ovis aries-derived cells has led to a relative lag in the study of its natural immunity and pathogenesis. Here we report the antiviral properties of ZAP against SRMV, a single-stranded negative-stranded RNA virus of the genus Morbillivirus. ZAP expression was significantly induced in sheep endometrial epithelial cells following SRMV infection. ZAP inhibited SRMV replication in cells after infection, while its overexpression in Vero-SLAM cells significantly increased their resistance to SRMV replication. The ZAP protein co-localized with SRMV RNA in the cytoplasm and ZAP-responsive elements were mapped to the 5' untranslated region of SRMV nucleocapsid, phosphoprotein, matrix, and fusion. In summary, ZAP confers resistance to SRMV infection by directly targeting viral RNA and inhibiting viral replication. Our findings further extend the ranges of viral targets of ZAP and help elucidate the mechanism of SRMV replication.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Chlorocebus aethiops
Endometrium virology
Epithelial Cells virology
Female
HEK293 Cells
Humans
Morbillivirus Infections virology
RNA, Viral genetics
RNA-Binding Proteins genetics
Sheep
Vero Cells
Virus Replication
Morbillivirus physiology
Morbillivirus Infections veterinary
RNA-Binding Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2542
- Volume :
- 260
- Database :
- MEDLINE
- Journal :
- Veterinary microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 34311269
- Full Text :
- https://doi.org/10.1016/j.vetmic.2021.109163