Back to Search
Start Over
Inhibition of acetic acid-induced colitis in rats by new Pediococcus acidilactici strains, vitamin producers recovered from human gut microbiota.
- Source :
-
PloS one [PLoS One] 2021 Jul 26; Vol. 16 (7), pp. e0255092. Date of Electronic Publication: 2021 Jul 26 (Print Publication: 2021). - Publication Year :
- 2021
-
Abstract
- Our aim was to isolate, identify and characterize probiotic bacteria as vitamin producers in particular B2 and B9. 150 human fecal samples were collected and used for isolation of vitamin producers-probiotics. 49 isolates were chosen for screening their genome by PCR for the presence of riboflavin and folic acid genes. As a result, three isolates were selected and their production of the B2 and B9 were confirmed by HPLC. The three isolates were identified on species level by sequencing their 16S rRNA gene which showed 100% identical to strains of Pediococcus acidilactici. Thus, they were named as P. acidilactici WNYM01, P. acidilactici WNYM02, P. acidilactici WNYM03 and submitted to the Genbank database with accession numbers. They met the probiotic criteria by expressing 90-95% survival rate at pH (2.0-9.0) and bile salt up to 2% for 3 h in addition to their antimicrobial activity against gram positive and negative microorganisms. They also showed no hemolytic activity and common pattern for antibiotic susceptibility. Our three strains were tested individually or in mixture in vivo on rat colitis model compared to ulcerative group. The strains were administrated orally to rats in daily dose containing CFU 109 for 14 days then followed by induction of colitis using acetic acid then the oral administration was continued for more four days. The histology results, the anti-inflammatory and anti-oxidative stress biomarkers showed the protective role of the strains compared to the ulcerative group. As a conclusion, we introduce novel three probiotic candidates for pharmaceutical preparations and health applications.<br />Competing Interests: The authors have declared that no competing interests exist.
- Subjects :
- Acetic Acid toxicity
Administration, Oral
Animals
Anti-Bacterial Agents pharmacology
Bile Acids and Salts pharmacology
Colitis chemically induced
Colon pathology
Disease Models, Animal
Feces microbiology
Folic Acid analysis
Humans
Hydrogen-Ion Concentration
Male
Pediococcus acidilactici drug effects
Pediococcus acidilactici genetics
Pediococcus acidilactici isolation & purification
RNA, Ribosomal, 16S analysis
RNA, Ribosomal, 16S metabolism
Rats
Rats, Wistar
Riboflavin analysis
Colitis therapy
Folic Acid metabolism
Gastrointestinal Microbiome
Pediococcus acidilactici physiology
Probiotics administration & dosage
Riboflavin metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 16
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 34310635
- Full Text :
- https://doi.org/10.1371/journal.pone.0255092