RNA-Sequencing Analysis of Differentially Expressed Genes in Human iPSC-Derived Cardiomyocytes.

Authors :: Okubo C
Narita M
Yamamoto T
Yoshida Y
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2320, pp. 193-217.
Publication Year :: 2021
Abstract: RNA sequencing profiles and characterizes cell and tissue samples, giving important insights into molecular mechanisms. Such data is imperative for cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) and used in related translational and basic research. Here we provide reliable protocols to extract differentially expressed genes in iPSC-CMs with RNA sequencing.<br /> (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)

Subjects :: Cell Differentiation genetics
Cells, Cultured
Chromosome Mapping
Gene Expression Profiling
Gene Library
Genes, Reporter
Genome, Human
Humans
MicroRNAs genetics
Principal Component Analysis
RNA isolation & purification
Sequence Alignment
Software
Induced Pluripotent Stem Cells cytology
Myocytes, Cardiac chemistry
RNA-Seq methods
Transcriptome

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