Staphylococcus caledonicus sp. nov. and Staphylococcus canis sp. nov. isolated from healthy domestic dogs.

Two strains, H8/1 ^T and H16/1A ^T , of Gram-stain-positive, coagulase-negative staphylococci were isolated from separate healthy domestic dogs in Scotland. Both strains were genome sequenced and their inferred DNA-DNA hybridisation indicates that H8/1 ^T and H16/1A ^T represent two novel species of the genus Staphylococcus . On the basis of the results of genome sequence analysis (genome blast distance phylogeny and single nucleotide polymorphism analysis) H8/1 ^T is most closely related to Staphylococcus devriesei and H16/1A ^T most closely related to Staphylococcus felis . Also, average nucleotide identity distinguished H8/1 ^T and H16/1A ^T from S. devriesei and S. felis as did minor phenotypic differences. On the basis of these results, it is proposed that H8/1 ^T and H16/1A ^T represent novel species with the respective names Staphylococcus caledonicus and Staphylococcus canis . The type strain of S. caledonicus is H8/1 ^T (=NCTC 14452 ^T =CCUG 74789 ^T ). The type strain of S. canis is H16/1A ^T (=NCTC 14451 ^T =CCUG 74790 ^T ).