Algoriphagus pacificus sp. nov. and Algoriphagus oliviformis sp. nov., isolated from a mariculture fishpond.

Two Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, non-flagellated, non-spore-forming and non-motile strains (YJ13C ^T and H41 ^T ) were isolated from a mariculture fishpond in PR China. Comparisons based on 16S rRNA gene sequences indicated that YJ13C ^T and H41 ^T shared 16S rRNA gene sequences similarities between 92.6 and 99.2 % with species of the genus Algoriphagus . YJ13C ^T only shared 93.8 % 16S rRNA gene sequence similarity with H41 ^T . The reconstructed phylogenetic and phylogenomic trees indicated that YJ13C ^T and H41 ^T clustered closely with species of the genus Algoriphagus . The calculated pairwise orthologous average nucleotide identity with usearch (OrthoANIu) values between strains YJ13C ^T and H41 ^T and other related strains were all less than 79.5 %. The OrthoANIu value between YJ13C ^T and H41 ^T was only 69.9 %. MK-7 was the predominant respiratory quinone of YJ13C ^T and H41 ^T and their major cellular fatty acids contained iso-C _15 : 0 , C _16 : 1 ω7 c and C _17 : 1 ω9 c . The polar lipids profiles of YJ13C ^T and H41 ^T consisted of phosphatidylethanolamine and several kinds of unidentified lipids. Combining the above descriptions, strains YJ13C ^T and H41 ^T represent two distinct novel species of the genus Algoriphagus , for which the names Algoriphagus pacificus sp. nov. (type strain YJ13C ^T =GDMCC 1.2178 ^T =KCTC 82450 ^T ) and Algoriphagus oliviformis sp. nov. (type strain H41 ^T =GDMCC 1.2179 ^T =KCTC 82451 ^T ) are proposed.