Involvement of Annexin A2 in Serum-MAF Dependent Phagocytic Activation of Macrophages.

Background/aim: Serum-derived macrophage activating factor (serum-MAF) is expected to have adjuvant effects through rapid phagocytic activation, which depends on F-actin accumulation in multi-layered membrane ruffles induced within 5 min after serum-MAF addition. This study aimed to elucidate the importance of annexin A2, which is a multifunctional Ca ²⁺ -binding protein related to cytoskeletal membrane dynamics, in serum-MAF signalling.
Materials and Methods: Annexin A2 and F-actin localizations were analyzed via immunostaining and confocal microscopy. Using EGTA as chelator, the role of Ca ²⁺ in serum-MAF signalling was examined.
Results: Annexin A2 was found to translocate from the cytosol to the cell cortex within 30 s of serum-MAF stimulation. Ca ²⁺ chelation inhibited the translocation of annexin A2, frill-like structure formation, and phagocytic activation by serum-MAF.
Conclusion: Annexin A2 and Ca ²⁺ were responsible for the rapid phagocytic activation by serum-MAF. This study provides an understanding of phagocytic activation in macrophages, which could be beneficial for cancer immunotherapy.
(Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)