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Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens.
- Source :
-
Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2021 Nov; Vol. 101 (3), pp. 115459. Date of Electronic Publication: 2021 Jun 20. - Publication Year :
- 2021
-
Abstract
- The performance of 2 antigenic commercial assays for enteric adenovirus (AdV) infection, bioNexia Rota-Adeno ImmunoChromatographic Tests (ICT) and LIAISON® Adenovirus ChemiLuminescence Immuno Assays (CLIA), was evaluated on 321 stools from children hospitalized for acute gastroenteritis in Palermo, Italy, using a Real time-PCR (Rt-PCR) as reference method. The CLIA showed higher sensitivity (77% vs 60%), accuracy (94.4 vs 90.9) and concordance (k: 0.81 vs 0.67) with respect to ICT, despite equivalent specificity (98.8%). Using the Ct values of the Rt-PCR as a proxy of the fecal viral load, similar Ct values (mean 9.32 vs 9.89) were observed among the true positive samples, whilst a significant difference (P < 0.05) was observed in false negative samples of CLIA (mean Ct 25.68) and ICT (mean Ct 19.87). Cross-reactivity with other enteric viruses was not observed. These results indicate that both the assays tested are suitable for diagnosis of AdV gastroenteritis.<br />Competing Interests: Conflict of interest The authors declare that they have no conflict of interest.<br /> (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Subjects :
- Adenoviridae genetics
Adolescent
Antigens, Viral genetics
Child
Child, Preschool
Feces virology
Hospitalization
Humans
Infant
Infant, Newborn
Italy
Luminescent Measurements methods
Sensitivity and Specificity
Adenoviridae immunology
Adenoviridae Infections diagnosis
Antigens, Viral immunology
Gastroenteritis diagnosis
Gastroenteritis virology
Luminescent Measurements standards
Reagent Kits, Diagnostic standards
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0070
- Volume :
- 101
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Diagnostic microbiology and infectious disease
- Publication Type :
- Academic Journal
- Accession number :
- 34280672
- Full Text :
- https://doi.org/10.1016/j.diagmicrobio.2021.115459