Oat (Avena sativa L.) Anther Culture.

Authors :: Warchoł M
Dziurka K
Czyczyło-Mysza I
Juzoń K
Marcińska I
Skrzypek E
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2287, pp. 313-322.
Publication Year :: 2021
Abstract: Production of doubled haploids (DHs) by androgenesis is a promising and convenient alternative to traditionally used breeding techniques. Low response of anther culture and strong genotype dependency in the development of embryo-like structures (ELS) was reported for oat (Avena sativa L.). Total homozygosity has been reached in one generation. This chapter describes a step-by-step protocol that can be useful for androgenesis studies and oat DH line production through anther culture.<br /> (© 2021. Springer Science+Business Media, LLC, part of Springer Nature.)