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Male heterogametic sex determination in Rana dybowskii based on sex-linked molecular markers.
- Source :
-
Integrative zoology [Integr Zool] 2022 Jan; Vol. 17 (1), pp. 105-114. Date of Electronic Publication: 2021 Jul 30. - Publication Year :
- 2022
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Abstract
- Identifying the mechanism for sex determination in amphibians is challenging. Very little is known about sex determination mechanisms of Rana dybowskii, a species of importance to evolutionary and conservation biology. We screened for sex-linked molecular markers in R. dybowskii in China using target region amplification polymorphism with 2 fixed primers against the sequences of Dmrt1. We found 2 male-linked molecular markers in R. dybowskii, which were 222 bp and 261 bp long. The detection rates of 222 bp marker in males form Xinglong, Huadian, and Dandong were 93.79%, 69.64%, and 13.64%, respectively, while the rate in females from Huadian was 27.50%. Besides, the detection rates of 261 bp marker in the above 3 regions were only observed in males at the rate of 93.79%, 87.50%, and 32.73%, respectively. The inheritance patterns of sex-linked molecular markers showed that the 2 sex-linked molecular markers were heterozygous. Compared to the XY-male parent, progeny from XX-pseudo-male parent possessed lower sex reversal ratio at the same rearing temperature, and the proportion of female froglets from an XX-pseudo-male parent was more than 95% at low rearing temperature (15°C). Our findings suggest that R. dybowskii displays male heterogamety, and the 2 sex-linked molecular markers may have a guiding significance for the protection and utilization of R. dybowskii.<br /> (© 2021 The Authors. Integrative Zoology published by International Society of Zoological Sciences, Institute of Zoology/Chinese Academy of Sciences and John Wiley & Sons Australia, Ltd.)
Details
- Language :
- English
- ISSN :
- 1749-4877
- Volume :
- 17
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Integrative zoology
- Publication Type :
- Academic Journal
- Accession number :
- 34254736
- Full Text :
- https://doi.org/10.1111/1749-4877.12577