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Assessment of Antimicrobial Agents, Analgesics, and Epidermal Growth Factors-Embedded Anti-Adhesive Poly(Lactic-Co-Glycolic Acid) Nanofibrous Membranes: In vitro and in vivo Studies.
Assessment of Antimicrobial Agents, Analgesics, and Epidermal Growth Factors-Embedded Anti-Adhesive Poly(Lactic-Co-Glycolic Acid) Nanofibrous Membranes: In vitro and in vivo Studies.
- Source :
-
International journal of nanomedicine [Int J Nanomedicine] 2021 Jul 01; Vol. 16, pp. 4471-4480. Date of Electronic Publication: 2021 Jul 01 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Background: Postoperative tissue adhesion is a major concern for most surgeons and is a nearly unpreventable complication after abdominal or pelvic surgeries. This study explored the use of sandwich-structured antimicrobial agents, analgesics, and human epidermal growth factor (hEGF)-incorporated anti-adhesive poly(lactic-co-glycolic acid) nanofibrous membranes for surgical wounds.<br />Materials and Methods: Electrospinning and co-axial electrospinning techniques were utilized in fabricating the membranes. After spinning, the properties of the prepared membranes were assessed. Additionally, high-performance liquid chromatography and enzyme-linked immunosorbent assays were utilized in assessing the in vitro and in vivo liberation profiles of the pharmaceuticals and the hEGF from the membranes.<br />Results: The measured data suggest that the degradable anti-adhesive membranes discharged high levels of vancomycin/ceftazidime, ketorolac, and hEGF in vitro for more than 30, 24, and 27 days, respectively. The in vivo assessment in a rat laparotomy model indicated no adhesion in the peritoneal cavity at 14 days post-operation, demonstrating the anti-adhesive capability of the sandwich-structured nanofibrous membranes. The nanofibers also released effective levels of vancomycin, ceftazidime, and ketorolac for more than 28 days in vivo. Histological examination revealed no adverse effects.<br />Conclusion: The outcomes of this study implied that the anti-adhesive nanofibers with sustained release of antimicrobial agents, analgesics, and growth factors might offer postoperative pain relief and infection control, as well as promote postoperative healing of surgical wounds.<br />Competing Interests: The authors report no conflicts of interest in this work.<br /> (© 2021 Liu et al.)
- Subjects :
- Adhesiveness drug effects
Analgesics chemistry
Animals
Anti-Infective Agents chemistry
Humans
Rats
Surgical Wound physiopathology
Wound Healing drug effects
Analgesics pharmacology
Anti-Infective Agents pharmacology
EGF Family of Proteins metabolism
Membranes, Artificial
Nanofibers chemistry
Polylactic Acid-Polyglycolic Acid Copolymer chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1178-2013
- Volume :
- 16
- Database :
- MEDLINE
- Journal :
- International journal of nanomedicine
- Publication Type :
- Academic Journal
- Accession number :
- 34234437
- Full Text :
- https://doi.org/10.2147/IJN.S318083