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Effects of Epstein-Barr Virus Infection on the Response of Human Breast Cancer Cells to Nicotine.
- Source :
-
Anticancer research [Anticancer Res] 2021 Jul; Vol. 41 (7), pp. 3449-3458. - Publication Year :
- 2021
-
Abstract
- Background/aim: The purpose of our study was to test whether EBV infection affects the response of human breast cancer cells to nicotine. In addition, the underlying signaling mechanisms were evaluated.<br />Materials and Methods: EBV-infected MDA-MB-231 and LMP1-transfected MDA-MB-231 breast cancer cells were established. Reverse transcription-polymerase chain reaction and western blotting were performed to evaluate nicotine receptor expression. To verify the functional role and underlying signaling mechanism of nicotine receptor expression induced by EBV infection, morphologic analysis, and proliferation and inhibition assays were performed.<br />Results: Both EBV infection and LMP1 transfection increased cell proliferation and induced the up-regulation of α9-nAChR expression. Additionally, nicotine treatment induced tumorigenic activity in both EBV-infected and LMP1-transfected MDA-MB-231 breast cancer cells. Western blot and inhibitor assays showed that the nicotine-induced signaling was mediated through MAPK/ERK and AKT signaling pathways in EBV-infected and LMP1-transfected breast cancer cells, respectively.<br />Conclusion: These results suggest that EBV infection and EBV-related LMP1 may act as potential molecular targets for breast cancer risk associated with nicotine, and provide a novel insight into the mechanism of nicotine stimulation in EBV-positive breast cancer cells.<br /> (Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Subjects :
- Breast Neoplasms pathology
Cell Line, Tumor
Cell Proliferation drug effects
Female
Humans
Signal Transduction drug effects
Up-Regulation drug effects
Breast Neoplasms drug therapy
Breast Neoplasms virology
Epstein-Barr Virus Infections pathology
Herpesvirus 4, Human physiology
Nicotine pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1791-7530
- Volume :
- 41
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Anticancer research
- Publication Type :
- Academic Journal
- Accession number :
- 34230140
- Full Text :
- https://doi.org/10.21873/anticanres.15132