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Human umbilical cord mesenchymal stem cells-derived exosomal microRNA-181a retards nasopharyngeal carcinoma development by mediating KDM5C.
- Source :
-
Journal of cancer research and clinical oncology [J Cancer Res Clin Oncol] 2021 Oct; Vol. 147 (10), pp. 2867-2877. Date of Electronic Publication: 2021 Jul 04. - Publication Year :
- 2021
-
Abstract
- Objective: It has been studied that mesenchymal stem cells (MSCs)-derived exosomes could suppress tumor growth in nasopharyngeal carcinoma (NPC) and microRNA-181a (miR-181a) could mediate drug resistance in NPC. Focused on this work, the mechanism of human umbilical cord MSCs (hUC-MSCs)-derived exosomal miR-181a was explored in NPC cell progression.<br />Methods: NPC tissues and normal tissues were obtained from patients, and miR-181a and KDM5C expression was examined. hUC-MSCs-derived exosomes were extracted, identified and co-cultured with NPC cells (C666-1 and SUNE1). C666-1 cell progression in vitro and/or tumor growth in vivo were examined after incubation with exosomes, miR-181a or lysine-specific demethylase 5C (KDM5C). miR-181a and KDM5C expression were examined in NPC.<br />Results: miR-181a expression was reduced while KDM5C expression was elevated in NPC. hUC-MSCs-derived exosomes restrained NPC cell growth in vivo and in vitro. Depleting or restoring exosomal miR-181a promoted or delayed NPC cell progression. KDM5C silencing suppressed NPC cell progression.<br />Conclusion: This study concluded that hUC-MSCs-derived exosomal miR-181a retards NPC development via negatively modulating KDM5C, serving as a candidate reference for the therapy of NPC.<br /> (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Animals
Apoptosis
Biomarkers, Tumor genetics
Biomarkers, Tumor metabolism
Cell Movement
Cell Proliferation
Female
Histone Demethylases genetics
Humans
Male
Mice
Mice, Inbred BALB C
Mice, Nude
Middle Aged
Nasopharyngeal Neoplasms genetics
Nasopharyngeal Neoplasms metabolism
Nasopharyngeal Neoplasms pathology
Prognosis
Tumor Cells, Cultured
Umbilical Cord cytology
Xenograft Model Antitumor Assays
Exosomes genetics
Gene Expression Regulation, Neoplastic
Histone Demethylases metabolism
Mesenchymal Stem Cell Transplantation methods
Mesenchymal Stem Cells cytology
MicroRNAs genetics
Nasopharyngeal Neoplasms prevention & control
Subjects
Details
- Language :
- English
- ISSN :
- 1432-1335
- Volume :
- 147
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of cancer research and clinical oncology
- Publication Type :
- Academic Journal
- Accession number :
- 34218325
- Full Text :
- https://doi.org/10.1007/s00432-021-03684-6