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The Lab4P Consortium of Probiotics Attenuates Atherosclerosis in LDL Receptor Deficient Mice Fed a High Fat Diet and Causes Plaque Stabilization by Inhibiting Inflammation and Several Pro-Atherogenic Processes.
- Source :
-
Molecular nutrition & food research [Mol Nutr Food Res] 2021 Sep; Vol. 65 (17), pp. e2100214. Date of Electronic Publication: 2021 Jul 19. - Publication Year :
- 2021
-
Abstract
- Scope: Previous studies show that Lab4 probiotic consortium plus Lactobacillus plantarum CUL66 (Lab4P) reduces diet-induced weight gain and plasma cholesterol levels in C57BL/6J mice fed a high fat diet (HFD). The effect of Lab4P on atherosclerosis is not known and is therefore investigated.<br />Methods and Results: Atherosclerosis-associated parameters are analyzed in LDL receptor deficient mice fed HFD for 12 weeks alone or supplemented with Lab4P. Lab4P increases plasma HDL and triglyceride levels and decreases LDL/VLDL levels. Lab4P also reduces plaque burden and content of lipids and macrophages, indicative of dampened inflammation, and increases smooth muscle cell content, a marker of plaque stabilization. Atherosclerosis arrays show that Lab4P alters the liver expression of 19 key disease-associated genes. Lab4P also decreases the frequency of macrophages and T-cells in the bone marrow. In vitro assays using conditioned media from probiotic bacteria demonstrates attenuation of several atherosclerosis-associated processes in vitro such as chemokine-driven monocytic migration, proliferation of monocytes and macrophages, foam cell formation and associated changes in expression of key genes, and proliferation and migration of vascular smooth muscle cells.<br />Conclusion: This study provides new insights into the anti-atherogenic actions of Lab4P together with the underlying mechanisms and supports further assessments in human trials.<br /> (© 2021 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH.)
- Subjects :
- Animals
Atherosclerosis genetics
Atherosclerosis pathology
Bone Marrow Cells
Cholesterol blood
Culture Media, Conditioned pharmacology
Diet, High-Fat adverse effects
Gene Expression Regulation
Lactobacillus plantarum
Lipids blood
Male
Mice, Mutant Strains
Organ Size
Plaque, Atherosclerotic pathology
Receptors, LDL genetics
Spleen growth & development
Mice
Atherosclerosis therapy
Liver physiology
Plaque, Atherosclerotic therapy
Probiotics pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1613-4133
- Volume :
- 65
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Molecular nutrition & food research
- Publication Type :
- Academic Journal
- Accession number :
- 34216185
- Full Text :
- https://doi.org/10.1002/mnfr.202100214