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[Determination of four protease inhibitors in chicken by ultra performance liquid chromatography-tandem mass spectrometry].
- Source :
-
Se pu = Chinese journal of chromatography [Se Pu] 2020 Feb 08; Vol. 38 (2), pp. 212-217. - Publication Year :
- 2020
-
Abstract
- A method was developed for the determination of four protease inhibitors (saquinavir, ritonavir, nelfinavir and indinavir) in chicken using ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The samples were extracted by shaking with 30% (v/v) acetonitrile aqueous solution (containing 1% (v/v) trichloroacetic acid), and purified by using mixed-mode cationic-exchanger (MCX) cartridges. The samples were separated on a Luna <superscript>®</superscript> C8 column (150 mm×2 mm, 3 μm) using 0.2% (v/v) formic acid aqueous solution (containing 5 mmol/L ammonium acetate) and acetonitrile as the mobile phases with gradient elution. The determination was carried out by using an electrospray ion source in the positive and multiple-reaction monitoring (MRM) modes. The calibration curves showed good linearities in the range of 0.1-20.0 μg/L, and the correlation coefficients ( r <superscript>2</superscript> ) were greater than 0.99. The limits of quantification (LOQs, S/N =10) of the four protease inhibitors varied from 0.20 μg/kg to 0.90 μg/kg. At the spiked levels of 1.0, 2.0, and 10.0 μg/kg, the average recoveries of the four protease inhibitors were ranging from 69.0% to 106.0%. The intra-day and inter-day relative standard deviations (RSDs) were 2.2%-13.8% ( n =6) and 3.6%-14.6% ( n =3), respectively. The method is simple, efficient, sensitive and accurate, and it can be used to detect residues of saquinavir, ritonavir, nelfinavir and indinavir in chicken.
Details
- Language :
- Chinese
- ISSN :
- 1872-2059
- Volume :
- 38
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Se pu = Chinese journal of chromatography
- Publication Type :
- Academic Journal
- Accession number :
- 34213170
- Full Text :
- https://doi.org/10.3724/SP.J.1123.2019.04047