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Distribution of the Water-Soluble Astaxanthin Binding Carotenoprotein (AstaP) in Scenedesmaceae.
- Source :
-
Marine drugs [Mar Drugs] 2021 Jun 20; Vol. 19 (6). Date of Electronic Publication: 2021 Jun 20. - Publication Year :
- 2021
-
Abstract
- Photooxidative stress-inducible water-soluble astaxanthin-binding proteins, designated as AstaP, were identified in two Scenedesmaceae strains, Coelastrella astaxanthina Ki-4 and Scenedesmus obtusus Oki-4N; both strains were isolated under high light conditions. These AstaPs are classified as a novel family of carotenoprotein and are useful for providing valuable astaxanthin in water-soluble form; however, the distribution of AstaP orthologs in other microalgae remains unknown. Here, we examined the distribution of AstaP orthologs in the family Scenedesmaceae with two model microalgae, Chlamydomonas reinhardtii and Chlorella variabilis . The expression of AstaP orthologs under photooxidative stress conditions was detected in cell extracts of Scenedesmaceae strains, but not in model algal strains. Aqueous orange proteins produced by Scenedesmaceae strains were shown to bind astaxanthin. The protein from Scenedesmus costatus SAG 46.88 was purified. It was named ScosAstaP and found to bind astaxanthin. The deduced amino acid sequence from a gene encoding ScosAstaP showed 62% identity to Ki-4 AstaP. The expression of the genes encoding AstaP orthologs was shown to be inducible under photooxidative stress conditions; however, the production amounts of AstaP orthologs were estimated to be approximately 5 to 10 times lower than that of Ki-4 and Oki-4N.
- Subjects :
- Carrier Proteins chemistry
Carrier Proteins genetics
Carrier Proteins isolation & purification
Chlorophyta chemistry
Chlorophyta classification
Light
Scenedesmus chemistry
Scenedesmus classification
Scenedesmus metabolism
Solubility
Water
Xanthophylls chemistry
Xanthophylls isolation & purification
Xanthophylls metabolism
Carrier Proteins metabolism
Chlorophyta metabolism
Oxidative Stress physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1660-3397
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Marine drugs
- Publication Type :
- Academic Journal
- Accession number :
- 34203079
- Full Text :
- https://doi.org/10.3390/md19060349