Multiphysics modelling of electroporation under uni- or bipolar nanosecond pulse sequences.

A nonlinear dispersive multiphysics model of single-cell electroporation was proposed in this paper. The time-domain Debye model was utilised to describe the membrane dispersion while the dynamic pore radius function was deployed to modify the plasma membrane conductivity. The dynamic spatial distributions of the ion concentration were dominated by the Nernst-Planck function. First, a single nanosecond pulsed electric field was applied to verify our model and to explore the effects of dispersion and dynamic pore radius on the redistribution of the electric field. The dispersive membrane was found to increase the transmembrane potential, expedite the electroporation process, and weaken the membrane permeability; however, adding the dynamic pore radius function had the opposite effect on transmembrane potential and membrane permeability. The responses of the cells exposed to unipolar and bipolar nanosecond pulse sequences were subsequently simulated. During the application of unipolar pulse sequences, the pore radius and perforation area showed a step-like accumulation, and significant increases in the perforation area and intracellular ion concentration were observed with higher frequency pulse sequences and wider subpulse intervals. The bipolar cancellation effect was also observed in terms of membrane permeability and pore radius.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
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