Morphology and organization of the internal shell of Ariolimax californicus (Gastropoda; Stylommatophora), an asymmetric two-face biomineralized matrix.

A slug is a shell-less terrestrial gastropod mollusk. During evolution, slugs have lost their mineralized external shell but some of them have retained an internal shell (IS). Unlike external shells, which have been widely investigated, the ISs have been poorly studied. We report for the first time the compositional and complete morphological characterization of Ariolimax californicus' IS. According to literature, this shell calcifies and decalcifies depending on the animal's needs. Its composition is mostly organic, consisting of proteins and β-chitin. The internal shell is organized in layers and membranes in which CaCO ₃ crystal formation occurs in specific areas. In the two faces of the IS we observed different morphologies and aggregations of calcite bio-crystals along with a different organization of the organic matrix. Dorsally, the mineral forms a thick layer composed of misaligned crystal aggregates of large dimensions, separated by thin organic layers. This suggests a protective purpose and the use of this layer as a long-term calcium storage system. Ventrally, the mineral phase is organized in small crystal aggregates of comparable size, separated by thin organic layers, and quite aligned one to the other. The whole ventral mineral layer is covered by a membrane, identified as the hypostracum. This face is proposed to be a short-term calcium storage system. In vitro crystallization experiments suggest massive calcium ions sequestration from the solution for the precipitation of calcite crystals inside the organic matrix. In conclusion, this research provides new information on the dynamic of biomineralization on mollusk evolved in calcium-poor environments.
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