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The cGAS/STING-TBK1-IRF Regulatory Axis Orchestrates a Primitive Interferon-Like Antiviral Mechanism in Oyster.
- Source :
-
Frontiers in immunology [Front Immunol] 2021 Jun 08; Vol. 12, pp. 689783. Date of Electronic Publication: 2021 Jun 08 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Interferon (IFN) system is considered as the first defense line against viral infection, and it has been extensively studied in vertebrates from fish to mammals. In invertebrates, Vagos from arthropod and IFN-like protein ( Cg IFNLP) from Crassostrea gigas appeared to function as IFN-like antiviral cytokines. In the present study, the Cg IFNLP protein in hemocytes was observed to increase after Poly (I:C) stimulation. After Cg IFNLP was knocked down by RNAi, the mRNA expression of IFN-stimulated genes ( Cg ISGs) was significantly inhibited. Both cyclic GMP-AMP synthase ( Cg cGAS) and stimulator of interferon gene ( Cg STING) identified from oyster were able to recognize the double-stranded nucleic acid [Poly (I:C) and dsDNA] and expressed at high level after Poly (I:C) stimulation. The expression of Cg IFNLP and interferon regulatory factors ( Cg IRF1/8) and the nuclear translocation of Cg IRF8 were all suppressed in Cg cGAS-RNAi or Cg STING-RNAi oysters after Poly (I:C) stimulation. The expression level of Cg STING and TANK binding kinase1 ( Cg TBK1) did not decrease in Cg cGAS-RNAi oysters. After Cg STING was knocked down, the high expression of Cg TBK1 induced by Poly (I:C) was prevented significantly. These results indicated that there was a primitive IFN-like antiviral mechanism dependent on the cGAS/STING-TBK1-IRFs regulatory axis in mollusks, which was different from the classic cGAS-STING-TBK1 signal pathway in mammals.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2021 Qiao, Zong, Liu, Wu, Li, Wang and Song.)
- Subjects :
- Animals
Crassostrea drug effects
Crassostrea immunology
Crassostrea virology
DNA Viruses immunology
Host-Pathogen Interactions
Interferon Regulatory Factors genetics
Membrane Proteins genetics
Nucleotidyltransferases genetics
Poly I-C pharmacology
Protein Serine-Threonine Kinases genetics
Signal Transduction
Crassostrea enzymology
Immunity drug effects
Interferon Regulatory Factors metabolism
Membrane Proteins metabolism
Nucleotidyltransferases metabolism
Protein Serine-Threonine Kinases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 34168656
- Full Text :
- https://doi.org/10.3389/fimmu.2021.689783