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Bacteremia caused by Anaerococcus SPP: Is this an underdiagnosed infection?
- Source :
-
Anaerobe [Anaerobe] 2021 Aug; Vol. 70, pp. 102405. Date of Electronic Publication: 2021 Jun 18. - Publication Year :
- 2021
-
Abstract
- The objectives of this study were to report 10 episodes of clinically significant bacteremia caused by species of the genus Anaerococcus isolated between July 2018 and February 2021 from the microbiology laboratory of a tertiary hospital in Granada (Spain). None of the isolates were identified by MALDI-TOF MS, and the definitive species identification was performed by 16 S rRNA gene sequencing. No reference spectra of the Anaerococcus species were present in the MALDI-TOF MS database. Eight isolates were finally identified as A. octavius, one isolate as A. tetradius and the other as A. urinomassiliensis. The majority of these infections were seen in patients aged >70 years. Risk factors for anaerobic infection were observed in eight patients, especially diabetes mellitus, surgery, and the presence of cancer. Fever was present in all patients. Three patients died, but only one death was attributed to the infection. Mean detection time of positive blood cultures was 47.5 h (range 24-92 h). Antimicrobial susceptibility to penicillin, amoxicillin-clavulanate, imipenem, moxifloxacin, clindamycin, metronidazole, and piperacillin-tazobactam was tested using the gradient diffusion technique and EUCAST breakpoints (except for moxifloxacin). No resistance to amoxicillin-clavulanate, metronidazole, imipenem, or piperacillin-tazobactam was detected; however, the majority of isolates were resistant to clindamycin. When MALDI-TOF MS does not provide a correct identification at genus or species level, as in some isolates of Gram-positive anaerobic cocci, microbiologists should perform an additional confirmatory technique, such as gene sequencing analysis, to obtain a definitive diagnosis.<br />Competing Interests: Declaration of competing interest The authors declare no conflict of interest.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adult
Aged
Aged, 80 and over
Anti-Bacterial Agents pharmacology
Bacteremia drug therapy
Bacterial Typing Techniques
DNA, Bacterial genetics
Female
Firmicutes classification
Firmicutes drug effects
Firmicutes genetics
Gram-Positive Bacterial Infections diagnosis
Gram-Positive Bacterial Infections drug therapy
Humans
Male
Microbial Sensitivity Tests
Middle Aged
RNA, Ribosomal, 16S genetics
Retrospective Studies
Spain
Bacteremia diagnosis
Bacteremia microbiology
Firmicutes isolation & purification
Gram-Positive Bacterial Infections microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1095-8274
- Volume :
- 70
- Database :
- MEDLINE
- Journal :
- Anaerobe
- Publication Type :
- Academic Journal
- Accession number :
- 34153469
- Full Text :
- https://doi.org/10.1016/j.anaerobe.2021.102405