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Remote Photothermal Control of DNA Origami Assembly in Cellular Environments.
- Source :
-
Nano letters [Nano Lett] 2021 Jul 14; Vol. 21 (13), pp. 5834-5841. Date of Electronic Publication: 2021 Jun 16. - Publication Year :
- 2021
-
Abstract
- In situ synthesis of DNA origami structures in living systems is highly desirable due to its potential in biological applications, which nevertheless is hampered by the requirement of thermal activation procedures. Here, we report a photothermal DNA origami assembly method in near-physiological environments. We find that the use of copper sulfide nanoparticles (CuS NPs) can mediate efficient near-infrared (NIR) photothermal conversion to remotely control the solution temperature. Under a 4 min NIR illumination and subsequent natural cooling, rapid and high-yield (>80%) assembly of various types of DNA origami nanostructures is achieved as revealed by atomic force microscopy and single-molecule fluorescence resonance energy transfer analysis. We further demonstrate the in situ assembly of DNA origami with high location precision in cell lysates and in cell culture environments.
- Subjects :
- Copper
DNA
Sulfides
Nanoparticles
Phototherapy
Subjects
Details
- Language :
- English
- ISSN :
- 1530-6992
- Volume :
- 21
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Nano letters
- Publication Type :
- Academic Journal
- Accession number :
- 34133183
- Full Text :
- https://doi.org/10.1021/acs.nanolett.1c01821