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Transcriptional regulator XtgS is involved in iron transition and attenuates the virulence of Streptococcus agalactiae.
- Source :
-
Research in veterinary science [Res Vet Sci] 2021 Sep; Vol. 138, pp. 109-115. Date of Electronic Publication: 2021 Jun 09. - Publication Year :
- 2021
-
Abstract
- Streptococcus agalactiae (GBS) is an important pathogen that has increasingly received attention for its role in invasive infections and its broad host range. Research on the regulation of gene expression could illuminate GBS pathogenesis. We previously identified a novel transcriptional regulator XtgS, which is a negative regulator of GBS pathogenicity. Here, we demonstrate that XtgS overexpression significantly attenuated GBS virulence in zebrafish infection tests, and XtgS indirectly downregulated the transcription of two iron transport systems based on the results of transcriptomic analysis, electrophoretic mobility shift assays (EMSAs) and lacZ fusion assays. Subsequent studies verified that the inactivation of iron transport system 1 resulted in GBS excessive iron accumulation and attenuated virulence. Thus, we infer that the downregulation of iron transport system 1 caused by XtgS overexpression probably attenuates bacterial virulence, which partially clarifies the mechanism by which XtgS alleviates the pathogenesis. These findings provide new insights into the relationship between exogenous transcriptional regulation and bacterial virulence.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Bacterial Proteins metabolism
Streptococcal Infections microbiology
Transcription Factors metabolism
Virulence genetics
Bacterial Proteins genetics
Fish Diseases microbiology
Iron metabolism
Streptococcal Infections veterinary
Streptococcus agalactiae genetics
Streptococcus agalactiae pathogenicity
Transcription Factors genetics
Zebrafish
Subjects
Details
- Language :
- English
- ISSN :
- 1532-2661
- Volume :
- 138
- Database :
- MEDLINE
- Journal :
- Research in veterinary science
- Publication Type :
- Academic Journal
- Accession number :
- 34126449
- Full Text :
- https://doi.org/10.1016/j.rvsc.2021.06.009