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Mitochondrial Coenzyme Q10 Determination Via Isotope Dilution Liquid Chromatography -Tandem Mass Spectrometry.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2275, pp. 329-339. - Publication Year :
- 2021
-
Abstract
- Coenzyme Q10 (CoQ <subscript>10</subscript> ) is an essential part of the mitochondrial respiratory chain . Here, we describe an accurate and sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for determination of mitochondrial CoQ <subscript>10</subscript> in isolated mitochondria . In the assay, mitochondrial suspensions are spiked with CoQ <subscript>10</subscript> -[ <superscript>2</superscript> H <subscript>9</subscript> ] internal standard (IS), extracted with organic solvents and CoQ <subscript>10</subscript> quantified by LC-MS/MS using multiple reaction monitoring (MRM).
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2275
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34118048
- Full Text :
- https://doi.org/10.1007/978-1-0716-1262-0_21