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Development of Lentiviral Vectors Pseudotyped With Influenza B Hemagglutinins: Application in Vaccine Immunogenicity, mAb Potency, and Sero-Surveillance Studies.
- Source :
-
Frontiers in immunology [Front Immunol] 2021 May 24; Vol. 12, pp. 661379. Date of Electronic Publication: 2021 May 24 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Influenza B viruses (IBV) cause respiratory disease epidemics in humans and are therefore components of seasonal influenza vaccines. Serological methods are employed to evaluate vaccine immunogenicity prior to licensure. However, classical methods to assess influenza vaccine immunogenicity such as the hemagglutination inhibition assay (HI) and the serial radial hemolysis assay (SRH), have been proven to have many limitations. As such, there is a need to develop innovative methods that can improve on these traditional assays and provide advantages such as ease of production and access, safety, reproducibility, and specificity. It has been previously demonstrated that the use of replication-defective viruses, such as lentiviral vectors pseudotyped with influenza A hemagglutinins in microneutralization assays (pMN) is a safe and sensitive alternative to study antibody responses elicited by natural influenza infection or vaccination. Consequently, we have produced Influenza B hemagglutinin-pseudotypes (IBV PV) using plasmid-directed transfection. To activate influenza B hemagglutinin, we have explored the use of proteases in increasing PV titers via their co-transfection during pseudotype virus production. When tested for their ability to transduce target cells, the influenza B pseudotypes produced exhibit tropism for different cell lines. The pseudotypes were evaluated as alternatives to live virus in microneutralization assays using reference sera standards, mouse and human sera collected during vaccine immunogenicity studies, surveillance sera from seals, and monoclonal antibodies (mAbs) against IBV. The influenza B pseudotype pMN was found to effectively detect neutralizing and cross-reactive responses in all assays and shows promise as an effective and versatile tool in influenza research.<br />Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.<br /> (Copyright © 2021 Ferrara, Del Rosario, da Costa, Kinsley, Scott, Fereidouni, Thompson, Kellam, Gilbert, Carnell and Temperton.)
- Subjects :
- A549 Cells
Animals
Antibodies, Neutralizing immunology
Antibodies, Viral immunology
Antibody Specificity immunology
Dogs
Genetic Vectors genetics
Genetic Vectors immunology
HEK293 Cells
Hemagglutinin Glycoproteins, Influenza Virus classification
Hemagglutinin Glycoproteins, Influenza Virus genetics
Humans
Influenza B virus genetics
Influenza B virus physiology
Influenza, Human immunology
Influenza, Human prevention & control
Influenza, Human virology
Lentivirus genetics
Madin Darby Canine Kidney Cells
Neutralization Tests methods
Orthomyxoviridae Infections immunology
Orthomyxoviridae Infections prevention & control
Orthomyxoviridae Infections virology
Vaccination
Vaccine Potency
Antibodies, Monoclonal immunology
Hemagglutinin Glycoproteins, Influenza Virus immunology
Immunogenicity, Vaccine immunology
Influenza B virus immunology
Influenza Vaccines immunology
Lentivirus immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1664-3224
- Volume :
- 12
- Database :
- MEDLINE
- Journal :
- Frontiers in immunology
- Publication Type :
- Academic Journal
- Accession number :
- 34108964
- Full Text :
- https://doi.org/10.3389/fimmu.2021.661379