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Intraocular pressure and injection forces during intravitreal injection into enucleated porcine eyes.
- Source :
-
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V [Eur J Pharm Biopharm] 2021 Sep; Vol. 166, pp. 87-93. Date of Electronic Publication: 2021 Jun 06. - Publication Year :
- 2021
-
Abstract
- Injection of biological molecules into the intravitreous humor is of increasing interest for the treatment of posterior segment eye diseases such as age-related degenerative macular degeneration. The injection volume is limited by an increase in intraocular pressure (IOP) and 50-100 µL are typically used for most intravitreally (IVT) applied commercial products. Direct measurement of IOP is difficult and has not been studied dependent on solution properties and injection rates. We used an instrumental set-up to study IOP ex vivo using healthy enucleated porcine eyes. IOP was determined as a function of injection volume for viscosities between 1 and 100 mPas, injection rates of 0.1, 1, and 1.5 mL/min, and needle length and diameter (27/30G and 0.5/0.75″) using Dextran solutions. IOP increased exponentially for injection volumes larger than 100 µL. We did not observe differences in IOP dependent on viscosity, injection rate, and needle diameter. However, variability increased significantly for injection volumes larger than 100 µL and, unexpectedly, declined with higher viscosities. We demonstrate that the exponential increase in IOP is not reflected by injection force measurements for typical configurations that are used for IVT application. The present findings may guide injection volumes for intravitreal injection and inform injection force considerations during technical drug product development.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Dextrans pharmacology
Disease Models, Animal
Drug Delivery Systems methods
Equipment Design
Needles
Organ Size
Plasma Substitutes pharmacology
Swine
Intraocular Pressure
Intravitreal Injections instrumentation
Intravitreal Injections methods
Pharmaceutical Solutions chemistry
Pharmaceutical Solutions pharmacology
Posterior Eye Segment pathology
Posterior Eye Segment physiology
Retinal Diseases drug therapy
Retinal Diseases physiopathology
Viscosity
Subjects
Details
- Language :
- English
- ISSN :
- 1873-3441
- Volume :
- 166
- Database :
- MEDLINE
- Journal :
- European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
- Publication Type :
- Academic Journal
- Accession number :
- 34102300
- Full Text :
- https://doi.org/10.1016/j.ejpb.2021.06.001