Detection of bladder cancer with aberrantly fucosylated ITGA3.

Authors :: Islam MK
Syed P
Dhondt B
Gidwani K
Pettersson K
Lamminmäki U
Leivo J
Source :: Analytical biochemistry [Anal Biochem] 2021 Sep 01; Vol. 628, pp. 114283. Date of Electronic Publication: 2021 Jun 05.
Publication Year :: 2021
Abstract: We describe a simple, non-invasive assay to identify fucosylated-glycoisoform of integrin alpha-3 (ITGA3) directly from unprocessed urine. ITGA3 was detected directly from the urine of bladder cancer (BlCa) (n = 13) and benign prostatic hyperplasia (BPH) (n = 9) patients with the use of lectins coated on europium-doped-nanoparticles (Eu <superscript>3+</superscript> -NPs). Lectin Ulex europaeus agglutinin-I (UEA) showed enhanced binding with BlCa-derived ITGA3. The evaluation with individual samples showed that a glycovariant ITGA3-UEA assay could significantly discriminate BlCa from BPH patients (p = 0.007). The detection of aberrantly fucosylated-isoform of ITGA3 from urine can be used to distinguish BlCa from age-matched benign controls in a simple sandwich assay.<br /> (Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.)