Assessment of Mitochondrial Reactive Oxygen Species and Redox Regulation in Stem Cells.

Authors :: Thumiah-Mootoo M
Podinic T
Khacho M
Source :: Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2277, pp. 289-297.
Publication Year :: 2021
Abstract: Mitochondrial reactive oxygen species (mtROS) and redox regulation play an important role in stem cell maintenance and cell fate decisions. Although changes in mtROS and redox homeostasis represent a physiological mechanism to drive stem cell commitment and differentiation, dysregulation of this system can lead to defects in stem cell maintenance and regenerative capacity. This chapter explains the methods used to assess mitochondrial superoxide levels and redox regulation in stem cell populations.

Subjects :: Animals
Gene Expression Profiling methods
Mice
Muscle, Skeletal cytology
Organophosphorus Compounds chemistry
Oxidation-Reduction
Phenanthridines chemistry
Reactive Oxygen Species metabolism
Stem Cells physiology
Superoxide Dismutase genetics
Superoxides analysis
Superoxides metabolism
Uncoupling Protein 2 genetics
Mitochondria metabolism
Reactive Oxygen Species analysis
Stem Cells metabolism

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