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Genetic Variation between Triploid and Diploid Clarias gariepinus (Burchell, 1822) Using RAPD Markers.
- Source :
-
Veterinary sciences [Vet Sci] 2021 May 04; Vol. 8 (5). Date of Electronic Publication: 2021 May 04. - Publication Year :
- 2021
-
Abstract
- This study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish Clarias gariepinus (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in C. gariepinus . Thereafter, 80 RAPD markers were screened; out of which, three showed the highest percentage of polymorphism (i.e., OPB 16 = 71.43%; OPC 14 = 61.9%; OPD 12 = 75%). The results obtained showed genotype differences between triploid and diploid without overlapping. However, the development of a Sequence Characterized Amplified Region (SCAR) marker was not achievable because progenies of triploid and diploid C. gariepinus could not be differentiated based on a specific fragment. Consequently, the genetic distance showed high similarities for both treatments and the UPGMA-generated dendrogram could not separate the treatments into two distinct clusters. It was concluded that RAPD makers cannot be used to separate the ploidy status of fishes.
Details
- Language :
- English
- ISSN :
- 2306-7381
- Volume :
- 8
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Veterinary sciences
- Publication Type :
- Academic Journal
- Accession number :
- 34064306
- Full Text :
- https://doi.org/10.3390/vetsci8050075