Back to Search Start Over

Genetic Variation between Triploid and Diploid Clarias gariepinus (Burchell, 1822) Using RAPD Markers.

Authors :
Normala J
Okomoda VT
Mohd AA
Nur AA
Abol-Munafi AB
Md Sheriff S
Source :
Veterinary sciences [Vet Sci] 2021 May 04; Vol. 8 (5). Date of Electronic Publication: 2021 May 04.
Publication Year :
2021

Abstract

This study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish Clarias gariepinus (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in C. gariepinus . Thereafter, 80 RAPD markers were screened; out of which, three showed the highest percentage of polymorphism (i.e., OPB 16 = 71.43%; OPC 14 = 61.9%; OPD 12 = 75%). The results obtained showed genotype differences between triploid and diploid without overlapping. However, the development of a Sequence Characterized Amplified Region (SCAR) marker was not achievable because progenies of triploid and diploid C. gariepinus could not be differentiated based on a specific fragment. Consequently, the genetic distance showed high similarities for both treatments and the UPGMA-generated dendrogram could not separate the treatments into two distinct clusters. It was concluded that RAPD makers cannot be used to separate the ploidy status of fishes.

Details

Language :
English
ISSN :
2306-7381
Volume :
8
Issue :
5
Database :
MEDLINE
Journal :
Veterinary sciences
Publication Type :
Academic Journal
Accession number :
34064306
Full Text :
https://doi.org/10.3390/vetsci8050075