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WDR62 is required for centriole duplication in spermatogenesis and manchette removal in spermiogenesis.
- Source :
-
Communications biology [Commun Biol] 2021 May 31; Vol. 4 (1), pp. 645. Date of Electronic Publication: 2021 May 31. - Publication Year :
- 2021
-
Abstract
- WDR62 is a scaffold protein involved in centriole duplication and spindle assembly during mitosis. Mutations in WDR62 can cause primary microcephaly and premature ovarian insufficiency. We have generated a genetrap mouse model deficient in WDR62 and characterised the developmental effects of WDR62 deficiency during meiosis in the testis. We have found that WDR62 deficiency leads to centriole underduplication in the spermatocytes due to reduced or delayed CEP63 accumulation in the pericentriolar matrix. This resulted in prolonged metaphase that led to apoptosis. Round spermatids that inherited a pair of centrioles progressed through spermiogenesis, however, manchette removal was delayed in WDR62 deficient spermatids due to delayed Katanin p80 accumulation in the manchette, thus producing misshapen spermatid heads with elongated manchettes. In mice, WDR62 deficiency resembles oligoasthenoteratospermia, a common form of subfertility in men that is characterised by low sperm counts, poor motility and abnormal morphology. Therefore, proper WDR62 function is necessary for timely spermatogenesis and spermiogenesis during male reproduction.
- Subjects :
- Animals
Cell Cycle genetics
Cell Cycle Proteins genetics
Centrioles metabolism
Cytoskeleton metabolism
Female
Male
Meiosis
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins genetics
Spermatids metabolism
Spermatogenesis physiology
Spermatozoa metabolism
Testis metabolism
Cell Cycle Proteins metabolism
Centrioles genetics
Nerve Tissue Proteins metabolism
Spermatogenesis genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2399-3642
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Communications biology
- Publication Type :
- Academic Journal
- Accession number :
- 34059773
- Full Text :
- https://doi.org/10.1038/s42003-021-02171-5