Back to Search
Start Over
Quantification of the inflammatory responses to pro-and anti-inflammatory agents in Manila clam, Ruditapes philippinarum.
- Source :
-
Fish & shellfish immunology [Fish Shellfish Immunol] 2021 Aug; Vol. 115, pp. 22-26. Date of Electronic Publication: 2021 May 28. - Publication Year :
- 2021
-
Abstract
- Inflammation is a form of innate immune response of living organisms to harmful stimuli. In marine bivalves, inflammation is a common defense mechanism. Several studies have investigated the morphological features of inflammation in bivalves, such as hemocyte infiltration. However, the molecular and biochemical responses associated with inflammation in marine bivalves remain unexplored. Here, we investigated changes in nitric oxide (NO) levels, cyclooxygenase 2 (COX-2) activity, and allograft inflammatory factor-1 (AIF-1) gene expression levels in hemolymph samples collected from Manila clam (Ruditapes philippinarum) exposed to pro- and anti-inflammatory substances. These included the pro-inflammatory agent lipopolysaccharide (LPS), and the nonsteroidal anti-inflammatory drugs (NSAIDs) ibuprofen and diclofenac, all widely used in vertebrates. Our study showed that NO levels, COX-2 activity, and AIF-1 expression increased in response to the treatments with LPS and decreased in response to the treatments with NSAIDs in a concentration-dependent manner. These results suggest that the mechanism of inflammatory responses in bivalves is very similar to that of vertebrates, and we propose that inflammatory responses can be quantified using these techniques and used to determine the physiological status of marine bivalves exposed to biotic or abiotic stresses.<br /> (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Calcium-Binding Proteins immunology
Cyclooxygenase 2 immunology
Diclofenac administration & dosage
Ibuprofen administration & dosage
Lipopolysaccharides administration & dosage
Nitric Oxide immunology
Water Pollutants, Chemical administration & dosage
Bivalvia genetics
Bivalvia immunology
Gene Expression immunology
Immunity, Innate genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1095-9947
- Volume :
- 115
- Database :
- MEDLINE
- Journal :
- Fish & shellfish immunology
- Publication Type :
- Academic Journal
- Accession number :
- 34052388
- Full Text :
- https://doi.org/10.1016/j.fsi.2021.05.019