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Hippocampal asymmetry and regional dispersal of nAChRs alpha4 and alpha7 subtypes in the adult rat.
- Source :
-
Journal of chemical neuroanatomy [J Chem Neuroanat] 2021 Oct; Vol. 116, pp. 101977. Date of Electronic Publication: 2021 May 27. - Publication Year :
- 2021
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Abstract
- To better comprehend the relationship between left/right (L/R) differences and hippocampus functions is necessary knowledge of lateral asymmetry and regional distribution. This research was design to examine hippocampal L/R asymmetry and regional distribution profile of the alpha7 and alpha4 subtypes of nicotinic acetylcholine receptors (nAChRs) in the adult rat. 10-12-week-old twenty-four male wistar rats were randomly selected. After removing the brains, immunohistochemistry, real-time PCR, and western blot methods were applied to distinguish the presence of the receptors in the hippocampus. Outcomes stated that the mentioned receptors expression profile was spatial-dependent. As, the hippocampal dispersal of alpha7 and alpha4 subtypes in the left hippocampus (LH) was remarkably maximum compare with the right hippocampus (RH) (p = 0.001, p = 0.005 respectively). Furthermore, the alpha7 optical density (OD) was not significantly different in the diverse regions in hippocampus of adult rat (p = 0.057), while the maximum OD of the alpha4 was detected in the hippocampal dentate gyrus and CA3 regions of LH (p = 0.007, p = 0.009 respectively) and the minimum OD was in the CA1 of the RH (p = 0.019). In real time PCR evaluation, there is a significantly higher expression of alpha7 and alpha4 in LH compared to RH (p = 0.043, p = 0.049 respectively), also, for western blot (p = 0.042, p = 0.030 respectively). According to present data, the alpha7 and alpha4 nAChR subtypes expression profile demonstrated lateral asymmetry, the uniform regional dispersal for alpha7 and different regional dispersal for alpha4 in the adult rat hippocampus.<br /> (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Hippocampus chemistry
Hippocampus cytology
Male
Rats
Rats, Wistar
Receptors, Nicotinic analysis
alpha7 Nicotinic Acetylcholine Receptor analysis
Functional Laterality physiology
Hippocampus metabolism
Receptors, Nicotinic biosynthesis
alpha7 Nicotinic Acetylcholine Receptor biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6300
- Volume :
- 116
- Database :
- MEDLINE
- Journal :
- Journal of chemical neuroanatomy
- Publication Type :
- Academic Journal
- Accession number :
- 34052301
- Full Text :
- https://doi.org/10.1016/j.jchemneu.2021.101977