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Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2274, pp. 155-168. - Publication Year :
- 2021
-
Abstract
- Sirtuins (SIRTs) are a family of NAD <superscript>+</superscript> -dependent histone deacetylases (HDACs). In mammals, dysfunction of SIRTs is associated with age-related metabolic diseases, cancers, and even aging. Therefore, the detection of SIRT activity in living cells or tissues would be helpful for diagnosis of a wide range of SIRT-associated diseases. Here, we present methodology to measure SIRT activity in living cells by using our newly developed SIRT fluorescence probe, KST-F-DA.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 2274
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 34050470
- Full Text :
- https://doi.org/10.1007/978-1-0716-1258-3_14