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Nanozyme based on graphene oxide modified with Fe 3 O 4 , CuO, and cucurbit[6]uril for colorimetric determination of homocysteine.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2021 May 28; Vol. 188 (6), pp. 207. Date of Electronic Publication: 2021 May 28. - Publication Year :
- 2021
-
Abstract
- A nanozyme based on graphene oxide modified with Fe <subscript>3</subscript> O <subscript>4</subscript> NPs, CuO NPs, and cucurbit[6]uril has been successfully fabricated by a simple sonochemical technique. By employing CB[6] as a specific binding pocket and Fe <subscript>3</subscript> O <subscript>4</subscript> @CuO-GO as a peroxidase mimic, this novel nanozyme (BN I) is equipped with molecular recognition ability and enhanced peroxidase-like activity. On the basis of the inhibition effect of homocysteine (Hcy) towards the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) catalyzed by BN I, a simple colorimetric method is established for the sensitive and selective determination of Hcy. This proposed method displays a good linear response in the range 5-200 μM with a detection limit of 1.8 μM. In the practical assay of human plasma samples, the relative standard deviations (RSD) are lower than 11% and the recoveries are between 98.0 and 104.9%. In the assay of human urine samples, the RSD are below 9.0% and the recoveries range from 94.0 to 103.5%. The colorimetric method presented offers a convenient and accurate way for the determination of biomarkers in point-of-care testing (POCT).
- Subjects :
- Biomimetic Materials chemistry
Catalysis
Homocysteine blood
Humans
Limit of Detection
Peroxidase chemistry
Peroxidase metabolism
Reproducibility of Results
Bridged-Ring Compounds chemistry
Colorimetry methods
Copper chemistry
Ferrosoferric Oxide chemistry
Graphite chemistry
Homocysteine analysis
Imidazoles chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 188
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 34047863
- Full Text :
- https://doi.org/10.1007/s00604-021-04868-0