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Fluorescent hollow ZrO 2 @CdTe nanoparticles-based lateral flow assay for simultaneous detection of C-reactive protein and troponin T.
- Source :
-
Mikrochimica acta [Mikrochim Acta] 2021 May 28; Vol. 188 (6), pp. 209. Date of Electronic Publication: 2021 May 28. - Publication Year :
- 2021
-
Abstract
- Highly fluorescent hollow ZrO <subscript>2</subscript> @CdTe nanoparticles (NPs) were synthesized efficiently via the hydrothermal method. By changing the hydrothermal time of ZrO <subscript>2</subscript> @CdTe NP, the peaks of fluorescence spectra measured at fluorescent excitation of 330 nm were at 540 nm, 590 nm, and 640 nm, respectively. Hollow ZrO <subscript>2</subscript> NPs have a uniform core-shell structure with the size of 178 ± 10 nm and shell of 19 ± 4 nm. The as-prepared yellow-ZrO <subscript>2</subscript> @CdTe NPs were used to develop lateral flow assay (LFA) for the sensitive and qualitative detection of C-reactive protein (CRP). The visual limit of detection of the LFA for the CRP antigen was 1 μg/L within 20 min, which is 1000-fold lower than that of colloidal gold-based LFA. In addition, a multiplex lateral flow assay (mLFA) was developed using the as-prepared green and red-ZrO <subscript>2</subscript> @CdTe NPs for the simultaneous, specific, sensitive, and qualitative detection of CRP and troponin T (cTnT). The visual limits of detection of CRP and cTnT in mLFA were 10 μg/L and 0.1 mg/L, respectively. The excellent performance of ZrO <subscript>2</subscript> @CdTe NPs should facilitate their application in point-of-care technology for the detection of other biomarkers.
- Subjects :
- Antibodies, Immobilized chemistry
Antibodies, Immobilized immunology
C-Reactive Protein immunology
Cadmium Compounds chemistry
Chromatography, Thin Layer
Fluorescent Dyes chemistry
Immunoassay
Limit of Detection
Tellurium chemistry
Troponin T immunology
Zirconium chemistry
C-Reactive Protein analysis
Nanoparticles chemistry
Spectrometry, Fluorescence methods
Troponin T analysis
Subjects
Details
- Language :
- English
- ISSN :
- 1436-5073
- Volume :
- 188
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Mikrochimica acta
- Publication Type :
- Academic Journal
- Accession number :
- 34047819
- Full Text :
- https://doi.org/10.1007/s00604-021-04865-3