Screening for Arabidopsis mutants with altered Ca 2+ signal response using aequorin-based Ca 2+ reporter system.

Environmental stimuli evoke transient increases of the cytosolic Ca ²⁺ level. To identify upstream components of Ca ²⁺ signaling, we have optimized two forward genetic screening systems based on Ca ²⁺ reporter aequorin. AEQ sig6 and AEQ ub plants were used for generating ethyl methanesulfonate (EMS)-mutagenized libraries. The AEQ sig6 EMS-mutagenized library was preferably used to screen the mutants with reduced Ca ²⁺ signal response due to its high effectiveness, while the AEQ ub EMS-mutagenized library was used for screening of the mutants with altered Ca ²⁺ signal response. For complete details on the use and execution of this protocol, please refer to Chen et al. (2020) and Zhu et al. (2013).
Competing Interests: The authors declare no competing interests.
(© 2021 The Authors.)