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AoATG5 plays pleiotropic roles in vegetative growth, cell nucleus development, conidiation, and virulence in the nematode-trapping fungus Arthrobotrys oligospora.
- Source :
-
Science China. Life sciences [Sci China Life Sci] 2022 Feb; Vol. 65 (2), pp. 412-425. Date of Electronic Publication: 2021 May 17. - Publication Year :
- 2022
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Abstract
- Autophagy is an evolutionarily conserved process in eukaryotes, which is regulated by autophagy-related genes (ATGs). Arthrobotrys oligospora is a representative species of nematode-trapping (NT) fungi that can produce special traps for nematode predation. To elucidate the biological roles of autophagy in NT fungi, we characterized an orthologous Atg protein, AoAtg5, in A. oligospora. We found that AoATG5 deletion causes a significant reduction in vegetative growth and conidiation, and that the transcript levels of several sporulation-related genes were significantly downregulated during sporulation stage. In addition, the cell nuclei were significantly reduced in the ΔAoATG5 mutant, and the transcripts of several genes involved in DNA biosynthesis, repair, and ligation were significantly upregulated. In ΔAoATG5 mutants, the autophagic process was significantly impaired, and trap formation and nematocidal activity were significantly decreased. Comparative transcriptome analysis results showed that AoAtg5 is involved in the regulation of multiple cellular processes, such as autophagy, nitrogen metabolism, DNA biosynthesis and repair, and vesicular transport. In summary, our results suggest that AoAtg5 is essential for autophagy and significantly contributes to vegetative growth, cell nucleus development, sporulation, trap formation, and pathogenicity in A. oligospora, thus providing a basis for future studies focusing on related mechanisms of autophagy in NT fungi.<br /> (© 2022. Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature.)
- Subjects :
- Animals
Ascomycota classification
Autophagosomes metabolism
Autophagy-Related Protein 5 genetics
Cell Nucleus genetics
DNA, Fungal metabolism
Fungal Proteins genetics
Gene Expression Profiling
Hyphae growth & development
Hyphae metabolism
Mutation
Nitrogen metabolism
Phylogeny
Spores, Fungal physiology
Transcription, Genetic
Virulence
Ascomycota pathogenicity
Ascomycota physiology
Autophagy-Related Protein 5 metabolism
Cell Nucleus metabolism
Fungal Proteins metabolism
Nematoda microbiology
Subjects
Details
- Language :
- English
- ISSN :
- 1869-1889
- Volume :
- 65
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Science China. Life sciences
- Publication Type :
- Academic Journal
- Accession number :
- 34031812
- Full Text :
- https://doi.org/10.1007/s11427-020-1913-9