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Using saturated absorption for superresolution laser scanning transmission microscopy.

Authors :
Nishida K
Sato H
Oketani R
Mochizuki K
Temma K
Kumamoto Y
Tanaka H
Fujita K
Source :
Journal of microscopy [J Microsc] 2022 Nov; Vol. 288 (2), pp. 117-129. Date of Electronic Publication: 2021 Jun 08.
Publication Year :
2022

Abstract

We improved the three-dimensional spatial resolution of laser scanning transmission microscopy by exploiting the saturated absorption of dye molecules. The saturated absorption is induced by the high-intensity light irradiation and localises the signal within the centre of the focal spot. Our numerical calculation indicates that the spatial resolution in transmission imaging is significantly improved for both lateral and axial directions using nonlinear transmitted signals induced by saturated absorption. We experimentally demonstrated the improvement of the three-dimensional resolution by observing fine structures of stained rat kidney tissues, which were not able to be visualised by conventional laser scanning transmission microscopy.<br /> (© 2021 Royal Microscopical Society.)

Details

Language :
English
ISSN :
1365-2818
Volume :
288
Issue :
2
Database :
MEDLINE
Journal :
Journal of microscopy
Publication Type :
Academic Journal
Accession number :
34028848
Full Text :
https://doi.org/10.1111/jmi.13033