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Synthesis of Profluorescent Strigolactone Probes for Biochemical Studies.

Authors :
de Saint Germain A
Clavé G
Boyer FD
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2309, pp. 219-231.
Publication Year :
2021

Abstract

In this chapter, we will describe a method we set up to synthesize two profluorescent strigolactone (SL) mimic probes (GC240 and GC242) and the optimized protocols developed to study the enzymatic properties of various strigolactone receptors. The Arabidopsis AtD14 SL receptor is used here as a model for this purpose.

Subjects

Subjects :
Arabidopsis drug effects
Arabidopsis genetics
Arabidopsis Proteins drug effects
Arabidopsis Proteins genetics
High-Throughput Screening Assays
Luminescent Measurements
Plant Growth Regulators pharmacology
Receptors, Cell Surface drug effects
Receptors, Cell Surface genetics
Signal Transduction
Arabidopsis metabolism
Arabidopsis Proteins metabolism
Biological Assay
Fluorescent Dyes chemical synthesis
Heterocyclic Compounds, 3-Ring metabolism
Lactones metabolism
Receptors, Cell Surface metabolism

Details

Language :
English
ISSN :
1940-6029
Volume :
2309
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
34028690
Full Text :
https://doi.org/10.1007/978-1-0716-1429-7_17
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