Biotechnological production of ssDNA with DNA-hydrolyzing deoxyribozymes.

Authors :: Liu J
Gu H
Source :: STAR protocols [STAR Protoc] 2021 May 10; Vol. 2 (2), pp. 100531. Date of Electronic Publication: 2021 May 10 (Print Publication: 2021).
Publication Year :: 2021
Abstract: Preparation of long single-stranded (ss)DNA in large quantities with high efficiency and purity remains a synthetic challenge. Here, we present a protocol for using DNA-hydrolyzing DNA enzymes (deoxyribozymes) for efficient biotechnological production of milligrams of ssDNA with a customizable sequence up to a few kilobases. Our protocol provides a convenient yet economical way to store the sequence information of target ssDNA on phages for selective mass production on demand. For complete details on the use and execution of this protocol, please refer to Jia et al. (2021).<br />Competing Interests: A China patent (ZLYY239-201032A) on large-scale production of ssDNA nanostructures with DNA-cleaving DNAs has been filed.<br /> (© 2021 The Author(s).)