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Live-cell imaging of glucose-induced metabolic coupling of β and α cell metabolism in health and type 2 diabetes.
- Source :
-
Communications biology [Commun Biol] 2021 May 19; Vol. 4 (1), pp. 594. Date of Electronic Publication: 2021 May 19. - Publication Year :
- 2021
-
Abstract
- Type 2 diabetes is characterized by β and α cell dysfunction. We used phasor-FLIM (Fluorescence Lifetime Imaging Microscopy) to monitor oxidative phosphorylation and glycolysis in living islet cells before and after glucose stimulation. In healthy cells, glucose enhanced oxidative phosphorylation in β cells and suppressed oxidative phosphorylation in α cells. In Type 2 diabetes, glucose increased glycolysis in β cells, and only partially suppressed oxidative phosphorylation in α cells. FLIM uncovers key perturbations in glucose induced metabolism in living islet cells and provides a sensitive tool for drug discovery in diabetes.
- Subjects :
- Animals
Diabetes Mellitus, Experimental drug therapy
Diabetes Mellitus, Experimental pathology
Diabetes Mellitus, Type 2 drug therapy
Diabetes Mellitus, Type 2 pathology
Glucagon-Secreting Cells drug effects
Glycolysis
Humans
Insulin-Secreting Cells drug effects
Islet Amyloid Polypeptide metabolism
Male
Mice
Microscopy, Fluorescence
Oxidative Phosphorylation
Rats
Rats, Sprague-Dawley
Rats, Transgenic
Diabetes Mellitus, Experimental metabolism
Diabetes Mellitus, Type 2 metabolism
Glucagon-Secreting Cells metabolism
Glucose pharmacology
Insulin-Secreting Cells metabolism
Molecular Imaging methods
Subjects
Details
- Language :
- English
- ISSN :
- 2399-3642
- Volume :
- 4
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Communications biology
- Publication Type :
- Academic Journal
- Accession number :
- 34012065
- Full Text :
- https://doi.org/10.1038/s42003-021-02113-1