Back to Search
Start Over
Time-Resolved and Label-Free Evanescent Light-Scattering Microscopy for Mass Quantification of Protein Binding to Single Lipid Vesicles.
- Source :
-
Nano letters [Nano Lett] 2021 Jun 09; Vol. 21 (11), pp. 4622-4628. Date of Electronic Publication: 2021 May 18. - Publication Year :
- 2021
-
Abstract
- In-depth understanding of the intricate interactions between biomolecules and nanoparticles is hampered by a lack of analytical methods providing quantitative information about binding kinetics. Herein, we demonstrate how label-free evanescent light-scattering microscopy can be used to temporally resolve specific protein binding to individual surface-bound (∼100 nm) lipid vesicles. A theoretical model is proposed that translates protein-induced changes in light-scattering intensity into bound mass. Since the analysis is centered on individual lipid vesicles, the signal from nonspecific protein binding to the surrounding surface is completely avoided, offering a key advantage over conventional surface-based techniques. Further, by averaging the intensities from less than 2000 lipid vesicles, the sensitivity is shown to increase by orders of magnitude. Taken together, these features provide a new avenue in studies of protein-nanoparticle interaction, in general, and specifically in the context of nanoparticles in medical diagnostics and drug delivery.
- Subjects :
- Light
Lipids
Protein Binding
Surface Plasmon Resonance
Microscopy
Nanoparticles
Subjects
Details
- Language :
- English
- ISSN :
- 1530-6992
- Volume :
- 21
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Nano letters
- Publication Type :
- Academic Journal
- Accession number :
- 34003003
- Full Text :
- https://doi.org/10.1021/acs.nanolett.1c00644