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IBSP, a potential recurrence biomarker, promotes the progression of colorectal cancer via Fyn/β-catenin signaling pathway.
- Source :
-
Cancer medicine [Cancer Med] 2021 Jun; Vol. 10 (12), pp. 4030-4045. Date of Electronic Publication: 2021 May 13. - Publication Year :
- 2021
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Abstract
- Colorectal cancer (CRC) is a frequently occurring digestive system cancer and postoperative tumor metastasis and recurrence are the main reasons for the failure of CRC treatment. The aim of this study was to identifying and validating key genes associated with metastatic recurrence of CRC. RNA expression of three datasets (GSE17538, GSE32323, and GSE29623) was used for biomarker discovery. We identified integrin-binding sialoprotein (IBSP) as a candidate biomarker which was validated in three clinical cohorts (GSE41258, GSE21510, and GSE39582) and our clinical specimens. The results suggested that IBSP expression significantly increased at mRNA and protein levels among CRC cases, which was associated with metastatic recurrence, metastasis, high risk of recurrence, and poor survival in CRC. Consistent results were obtained in CRC cells. The relative level of serum IBSP evidently increased among CRC patients relative to normal controls, and downregulated after operation. As suggested by gene set enrichment analysis (GSEA), the IBSP level was associated with cell-matrix adhesion in CRC. Functional experiments in vitro showed that IBSP promoted the growth and aggressiveness of CRC, and the potential mechanism by which IBSP promoted carcinogenesis of CRC was the abnormal activation of Fyn/β-catenin signaling pathway. To sum up, findings in the present work indicate that IBSP can serve as the candidate biomarker for the diagnosis, treatment, and prognosis of CRC.<br /> (© 2021 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
- Subjects :
- Apoptosis
Biomarkers, Tumor blood
Biomarkers, Tumor metabolism
Cell Proliferation
Colon metabolism
Colorectal Neoplasms metabolism
Colorectal Neoplasms mortality
Colorectal Neoplasms pathology
Databases, Genetic
Down-Regulation
Female
Humans
Integrin-Binding Sialoprotein blood
Integrin-Binding Sialoprotein metabolism
Male
Middle Aged
Neoplasm Recurrence, Local metabolism
Neoplasm Recurrence, Local mortality
Proto-Oncogene Proteins c-fyn metabolism
RNA metabolism
RNA, Messenger metabolism
beta Catenin metabolism
Biomarkers, Tumor genetics
Colorectal Neoplasms genetics
Disease Progression
Integrin-Binding Sialoprotein genetics
Neoplasm Recurrence, Local genetics
Subjects
Details
- Language :
- English
- ISSN :
- 2045-7634
- Volume :
- 10
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Cancer medicine
- Publication Type :
- Academic Journal
- Accession number :
- 33987980
- Full Text :
- https://doi.org/10.1002/cam4.3959