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Immunogenicity of replication-deficient vesicular stomatitis virus based rabies vaccine in mice.
- Source :
-
The veterinary quarterly [Vet Q] 2021 Dec; Vol. 41 (1), pp. 202-209. - Publication Year :
- 2021
-
Abstract
- Background: Rabies is a viral disease that causes severe neurological manifestations both in humans and various mammals. Although inactivated and/or attenuated vaccines have been developed and widely used around the world, there are still concerns with regard to their safety, efficacy, and costs.<br />Objective: As demand has grown for a new rabies vaccine, we have developed a new vesicular stomatitis viruses (VSVs) based rabies vaccine that replaces glycoproteins with rabies virus (RABV) glycoprotein (GP), or so-called VSV/RABV-GP.<br />Methods: VSV/RABV-GP production was measured by sandwich ELISA. The generation of VSV/RABV-GP was evaluated with GP-specific antibodies and reduced transduction with GP-specific neutralizing antibodies. Virus entry was quantified by measuring the luciferase levels at 18-h post-transduction. BALB/c mice (three groups of six mice each) were intraperitoneally immunized with PBS, RABA, or VSV/RABV-GP at 0 and 14 days. At 28 days post-immunization serology was performed. Statistical significance was calculated using the Holm-Sidak multiple Student's t test.<br />Results: Mice immunized with VSV/RABV-GP produced IgM and IgG antibodies, whereas IgM titers were significantly higher in mice immunized with VSV/RABV-GP compared to inactivated RABV. The secretion profiles of IgG1 and IgG2a production suggested that VSV/RAVB-GP induces the T helper cell type-2 immune bias. In addition, the average (±SD; n = 3) serum neutralization titers of the inactivated RABV and VSV/RABV-GP groups were 241 ± 40 and 103 ± 54 IU/mL, respectively.<br />Conclusion: Our results confirm that VSV/RABV-GP could be a new potential vaccination platform for RABV.
- Subjects :
- Animals
Antibodies, Neutralizing
Antibodies, Viral
Glycoproteins genetics
Immunoglobulin G
Immunoglobulin M
Mice, Inbred BALB C
Rabies immunology
Rabies virology
Rabies virus genetics
Vaccines, Synthetic
Viral Proteins
Virus Replication genetics
Mice
Glycoproteins immunology
Immunogenicity, Vaccine
Rabies Vaccines immunology
Rabies virus immunology
Vesiculovirus genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1875-5941
- Volume :
- 41
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- The veterinary quarterly
- Publication Type :
- Academic Journal
- Accession number :
- 33985418
- Full Text :
- https://doi.org/10.1080/01652176.2021.1930277